Long-Term Monitoring of Post-Stroke Plasticity After Transient Cerebral Ischemia in Mice Using In Vivo and Ex Vivo Diffusion Tensor MRI
C Granziera*, 1, 2, H D’Arceuil*, #, 1, L Zai3, P.J Magistretti4, A.G Sorensen1, A.J de Crespigny1
Identifiers and Pagination:Year: 2007
First Page: 10
Last Page: 17
Publisher Id: TONIJ-1-10
Article History:Received Date: 13/8/2007
Revision Received Date: 11/10/2007
Acceptance Date: 18/10/2007
Electronic publication date: 13/11/2007
Collection year: 2007
We used a murine model of transient focal cerebral ischemia to study: 1) in vivo DTI long-term temporal evolution of the apparent diffusion coefficient (ADC) and diffusion fractional anisotropy (FA) at days 4, 10, 15 and 21 after stroke 2) ex vivo distribution of a plasticity-related protein (GAP-43) and its relationship with the ex vivo DTI characteristics of the striato-thalamic pathway (21 days).
All animals recovered motor function. In vivo ADC within the infarct was significantly increased after stroke. In the stroke group, GAP-43 expression and FA values were significantly higher in the ipsilateral (IL) striatum and contralateral (CL) hippocampus compared to the shams. DTI tractography showed fiber trajectories connecting the CL striatum to the stroke region, where increased GAP43 and FA were observed and fiber tracts from the CL striatum terminating in the IL hippocampus.
Our data demonstrate that DTI changes parallel histological remodeling and recovery of function.